Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/106566
Type: Artigo de periódico
Title: Cytotoxicity Of Dehydrocrotonin (a Nor-clerodane From Croton Cajucara) On Human Lymphocytes
Author: Lemos T.M.A.M.
Aoyama H.
Cavagis A.D.M.
Abstract: Trans-Dehydrocrotonin, a 19-nor-clerodane, is the major norditerpene obtained from Croton cajucara, a Brazilian medicinal plant which presents important biological effects, such as antineoplastic and antiulcerogenic activities. In this work, we analyzed the effect of this sesquiterpene lactone on normal human lymphocytes. The cell viability was verified after treatment for 24 and 72 h with trans-dehydrocrotonin, in the presence and absence of phytohemagglutin (specific mitogen for this cell), through three end-points to assess cytotoxicity in vitro: MTT reduction (mitochondrial function), protein quantification (cell number) and phosphatase activity (cell metabolism). When the cells were treated with dehydrocrotonin in the presence of mitogen, no toxic effect was observed. Nevertheless, in the absence of mitogen, the IC50 was 450 μM for MTT reduction and phosphatase activity. Moreover, in this condition, trans-dehydrocrotonin caused stimulation of protein content from 100 μM. Our results suggest that phytohemagglutin protects human lymphocytes against the trans-dehydrocrotonin toxic effect.
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Rights: fechado
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Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-63249095822&partnerID=40&md5=3e18cf9a2153f90988d5f1917c8bd2d0
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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