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Type: Artigo de periódico
Title: Environmental Contamination And Enterotoxigenic Profile Of Bacillus Cereus Isolated In Food Services [contaminação Ambiental E Perfil Toxigênico De Bacillus Cereus Isolados Em Serviços De Alimentação]
Author: Soares C.M.
Valadares G.F.
De Azeredo R.M.C.
Kuaye A.Y.
Abstract: Ninety air samples and ninety six samples from benches and equipments surfaces were collected in two food services for investigation of Bacillus cereus contamination sources and characterization of strains toxin profiles. B. cereus was detected in 84.4% and 44.8% from air samples and samples from benches and equipments surfaces, respectively. The potential of enterotoxin production was investigated using polymerase chain reaction (PCR) methods for genes hblA, hblD e hblC (encoding hemolysin BL) and for genes nheA, nheB and nheC (encoding non-hemolytic enterotoxin - NHE). From 70 isolates investigated 14.3% were positive for the three HBL encoding genes and 12.8% were positive for the three NHE encoding genes. The Bacillus Diarrhoeal Enterotoxin Visual Immunoassay (BDE-VIA; Tecra) also was used for NHE detection. The results obtained with BDE-VIA revealed that 61.4% from the 70 strains are NHE producers.
Rights: aberto
Identifier DOI: 10.1590/S0103-84782008000200033
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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