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Type: Artigo de periódico
Title: Study Of Different Parameters In The Production Of Lytic Enzymes [estudo Da Influência De Diferentes Parâmetros Na Produção De Enzimas Líticas]
Author: Fleuri L.F.
Sato H.H.
Abstract: The aim of this work was to study the effects of different parameters in the production of β-1,3 glucanases, proteases and chitinases by Cellulosimicrobium celulans 191, in culture media A, B and C, respectively. The experimental designs employed were 23 factorial designs, and the factors studied were: initial pH, temperature and rotary shaker speed. The experimental results for the production of β-1,3 glucanase in culture medium A showed maximum activity (0.64 U.mL-1) with an initial pH of 8.5 after 24 hours of fermentation at 33°C and 200 rpm. The parameters pH, rotary shaker speed and the interaction of all the parameters presented statistical significance. The experimental results for protease production in culture medium B showed maximum activity (4.25 U.mL-1) with an initial pH of 6.5 after 30 hours of fermentation at 20°C and 200 rpm. The pH was the only parameter that presented statistical significance. The experimental results for chitinase production in culture medium C showed maximum activity (7.06 U.mL-1) at an initial pH of 5.5 after 72 hours of fermentation at 25°C and 200 rpm. All the parameters and their interactions presented statistical significance. The determination coefficients of the analysis of variance and F tests demonstrated that the linear regression models obtained were significant at a confidence level of 95%.
Rights: aberto
Identifier DOI: 10.1590/S0101-20612008000200006
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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