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Type: Artigo de periódico
Title: Validation Of Analytical Methodology By Hplc For Quantification Of Bupivacaine (s75-r25) In Poli-lactide-co-glicolide Nanospheres [validação De Metodologia Analítica Por Cromatografia Líquida De Alta Eficiência Para Quantificação De Bupivacaína (s75-r25) Em Nanoesferas De Poli(lactídeo- Coglicolídeo)]
Author: Moraes C.M.
De Paula E.
Rosa A.H.
Fraceto L.F.
Abstract: Bupivacaine (S75-R25, NovaBupi®) is an amide type local anesthetic widely used. The present work consists of the development and validation of analytical methodology for evaluation of NovaBupi® content in the poly-lactide-co-glycolide nanospheres (PLGA-NS) by high performance liquid chromatography. The separation was made using the reversed-phase column LC-18, acetonitrile/phosphate buffer 85:15 v/v as mobile phase and detection at 220 nm. The results obtained show that the analytical methodology is accurate, reproducible, robust and linear over the concentration range 10-220.0 μg/mL of NovaBupi®. The method was applied to determine the encapsulation efficiency and evaluate the release profile of NovaBupi®, showing good results.
Rights: aberto
Identifier DOI: 10.1590/S0100-40422008000800040
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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