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|Type:||Artigo de periódico|
|Title:||Validation Of Analytical Methodology By Hplc For Quantification Of Bupivacaine (s75-r25) In Poli-lactide-co-glicolide Nanospheres [validação De Metodologia Analítica Por Cromatografia Líquida De Alta Eficiência Para Quantificação De Bupivacaína (s75-r25) Em Nanoesferas De Poli(lactídeo- Coglicolídeo)]|
De Paula E.
|Abstract:||Bupivacaine (S75-R25, NovaBupi®) is an amide type local anesthetic widely used. The present work consists of the development and validation of analytical methodology for evaluation of NovaBupi® content in the poly-lactide-co-glycolide nanospheres (PLGA-NS) by high performance liquid chromatography. The separation was made using the reversed-phase column LC-18, acetonitrile/phosphate buffer 85:15 v/v as mobile phase and detection at 220 nm. The results obtained show that the analytical methodology is accurate, reproducible, robust and linear over the concentration range 10-220.0 μg/mL of NovaBupi®. The method was applied to determine the encapsulation efficiency and evaluate the release profile of NovaBupi®, showing good results.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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