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Type: Artigo de periódico
Title: Optimization Of A Method For Determination Of Flavonols And Flavones In Fruits By Hplc Using Statistical Design And Response Surface Analysis [otimização De Método Para Determinação De Flavonóis E Flavonas Em Frutas Por Cromatografia Líquida De Alta Eficiência Utilizando Delineamento Estatístico E Análise De Superfície De Resposta]
Author: Hoffmann-Ribani R.
Rodriguez-Amaya D.B.
Abstract: This work optimized the HPLC conditions for the simultaneous determination of luteolin, apigenin, myricetin, quercetin and kaempferol in aglycone form, as well defined the best conditions for hydrolysis/extraction of these flavonoids in fruits, using the statistical central composite design and response surface analysis. A reverse phase method was developed using a gradient of methanol/water acidified with 0.3% formic acid as mobile phase and a photodiode array detector. The samples were extracted with methanol/water (50:50 v/v) at 90̊C. The optimum time and HCl concentration varied for the different fruits investigated, demonstrating the necessity of optimizing these conditions for each fruit analyzed. Good recovery (87.1 to 96.3%), repeatability and linearity were obtained.
Rights: aberto
Identifier DOI: 10.1590/S0100-40422008000600020
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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