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Type: Artigo de periódico
Title: The Catalytic Mechanism Of Indole-3-glycerol Phosphate Synthase (igps) Investigated By Electrospray Ionization (tandem) Mass Spectrometry
Author: Czekster C.M.
Lapis A.A.M.
Souza G.H.M.F.
Eberlin M.N.
Basso L.A.
Santos D.S.
Dupont J.
Neto B.A.D.
Abstract: An enzymatic reaction has been monitored by on-line direct infusion electrospray ionization (tandem) mass spectrometry. Using this fast and sensitive technique, a key and transient intermediate of Mycobacterium tuberculosis indole-3-glycerol phosphate synthase (IGPS)-catalyzed reaction has been trapped. The reaction catalyzed by indole-3-glycerol phosphate synthase is part of the tryptophan biosynthetic pathway, and is not present in mammals, including humans. This peculiarity renders this enzyme a potential target for the development of biospecific agents with potential anti-TB activity. The present results indicate the presence of two intermediates in the mechanism of this enzymatic reaction. © 2008 Elsevier Ltd. All rights reserved.
Rights: fechado
Identifier DOI: 10.1016/j.tetlet.2008.07.149
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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