Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/105294
Type: Artigo de periódico
Title: Lymph Vascular Invasion In Invasive Mammary Carcinomas Identified By The Endothelial Lymphatic Marker D2-40 Is Associated With Other Indicators Of Poor Prognosis
Author: Marinho V.F.Z.
Metze K.
Sanches F.S.F.
Rocha G.F.S.
Gobbi H.
Abstract: Background: Immunohistochemical studies of lymphatic vessels have been limited by a lack of specific markers. Recently, the novel D2-40 antibody, which selectively marks endothelium of lymphatic vessels, was released. The aim of our study is to compare lymphatic and blood vessel invasion detected by hematoxylin and eosin (H&E) versus that detected by immunohistochemistry, relating them with morphologic and molecular prognostic factors. Methods: We selected 123 cases of invasive mammary carcinomas stratified into three subgroups according to axillary lymph node status: macrometastases, micrometastases, and lymph node negative. Lymphatic (LVI) and blood (BVI) vessel invasion were evaluated by H&E and immunohistochemistry using the D2-40 and CD31 antibodies, and related to histologic tumor type and grade, estrogen and progesterone receptors, E-cadherin, Ki67, p53, and Her2/neu expression. Results: LVI was detected in H&E-stained sections in 17/123 cases (13.8%), and in D2-40 sections in 35/123 cases (28.5%) (Kappa = 0.433). BVI was detected in H&E-stained sections in 5/123 cases (4.1%), and in CD31 stained sections in 19/123 cases (15.4%) (Kappa = 0.198). LVI is positively related to higher histologic grade (p = 0.013), higher Ki67 expression (p = 0.00013), and to the presence of macrometastases (p = 0.002), and inversely related to estrogen (p = 0.0016) and progesterone (p = 0.00017) receptors expression. Conclusion: D2-40 is a reliable marker of lymphatic vessels and is a useful tool for lymphatic emboli identification in immunostained sections of breast carcinomas with higher identification rates than H&E. Lymphatic vessel invasion was related to other features (high combined histologic grade, high Ki67 score, negative hormone receptors expression) associated with worse prognosis, probable reflecting a potential for lymphatic metastatic spread and aggressive behavior. © 2008 Marinho et al; licensee BioMed Central Ltd.
Editor: 
Rights: aberto
Identifier DOI: 10.1186/1471-2407-8-64
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-42349109578&partnerID=40&md5=16e0537cb1854ded5a30bd6eaac0448c
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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