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|Type:||Artigo de periódico|
|Title:||Expression Of Hypoxia-inducible Factor 1α In Mononuclear Phagocytes Infected With Leishmania Amazonensis|
|Abstract:||Increasing evidence indicates that hypoxia-inducible factor 1α (HIF-1α) can be upregulated in different cell types by nonhypoxic stimuli such as growth factors, cytokines, nitric oxide, lipopolysaccharides and a range of infectious microorganisms. In this study, the ability of the following mononuclear phagocytes to express HIF-1α is reported: mouse macrophages (mMΦ), human macrophages (hMΦ) and human dendritic cells (DC), parasitized in vitro with Leishmania amazonensis; as assessed by immunofluorescence microscopy. A logical explanation for HIF-1α expression might be that the mononuclear phagocytes became hypoxic after L. amazonensis infection. Using the hypoxia marker pimonidazole, observation revealed that L. amazonensis-infected cells were not hypoxic. In addition, experiments using a HIF-1α inhibitor, CdCl2, to treat L. amazonensis-infected macrophage cultures showed reduced parasite survival. These studies indicated that HIF-1α could play a role in adaptative and immune responses of mononuclear phagocytes presenting infection by the parasite L. amazonensis. © 2007 Elsevier B.V. All rights reserved.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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