Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/104609
Type: Artigo de periódico
Title: Study Of The Adhesion And Invasion Capacities Of An Invasive Avian Pathogenic Escherichia Coli Strain (apec) To In Vitro Cultivated Hep-2 Cells
Author: Nakazato G.
De Campos T.A.
Lago J.C.
Brocchi M.
De Castro A.F.P.
Stehling E.G.
Da Silveira W.D.
Abstract: An avian pathogenic Escherichia coli strain (APEC), designated strain sep17, was isolated from the liver of a chicken suffering from septicaemia. Its biological characteristics, including antimicrobial drug resistances, colicin production, plasmid profile, adhesion and invasion capacities to in vitro cultivated HEp-2 cells, and PCR detection of DNA sequences related to pathogenicity genes (fimA, tsh, papA, crl, csgA, afa, sfa, eae, lpfA O157/OI-141, lpfAO157/OI-154, toxB, iha, ial, efa, inv, invA/invE and ibeA) were determined. This strain (Lac+, Tc R, fimA, csgA, crl, lpfAO157/OI-154) harbored a 70 MDa plasmid and was able to adhere to and invade in vitro cultured HEp-2 cells. Transference of this 70 MDa plasmid by conjugation rendered a non-pathogenic recipient strain HB101 (Lac-, SmR, csgA) capable of adhering to and invading the same type of cell. Scanning electron microscopy and light microscopy confirmed the adhesion capacity of the wild and the transconjugant strains, while the in vitro invasion technique and light microscopy confirmed the invasion capacity of these strains. The FAS technique, which is used to visualize actin accumulation on cells where the adhesion process occurs, was negative for all those strains. None of the genes detected in strain sep17 were transferred by conjugation, which indicates that they are chromosomally located and are not related to the adhesion and invasion processes. The presence and absence of pathogenicity-related genes are discussed.
Editor: 
Rights: aberto
Identifier DOI: 
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-36448930558&partnerID=40&md5=dc5a1b6103703a11f5f6e2339bf97768
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.