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|Type:||Artigo de periódico|
|Title:||Dna Content And Chromatin Texture Of Human Breast Epithelial Cells Transformed With 17-β-estradiol And The Estrogen Antagonist Ici 182,780 As Assessed By Image Analysis|
|Abstract:||The immortalized human breast epithelial MCF-10F cell line, although estrogen receptor α negative, develops cell proliferating activities and invasiveness indicative of neoplastic transformation, after treatment with 17-β-estradiol (E-2). These effects are similar to those produced by benzo[a]pyrene (BP). Since we have previously reported changes in the nuclear parameters accompanying BP-induced tumorigenesis in MCF-10F cells, we have examined whether similar alterations occur in E-2-treated cells. We therefore studied DNA amounts and other nuclear parameters in Feulgen-stained MCF-10F cells after treatment with various concentrations of E-2, BP, the estrogen antagonist ICI 182,780, and E-2 in the presence of ICI 182,780. E-2 caused a certain loss of DNA and changes in the nuclear size and chromatin supraorganization of MCF-10F cells. Many of these changes were similar to those produced by BP and were indicative of neoplastic transformation. More intense chromatin remodelling was seen with 70 nM E-2. Since these changes were not abrogated totally or partially by ICI 182,780, the neoplastic transformation of MCF-10F cells stimulated by E-2 involved a process that was independent of estrogen α-receptors. The changes produced by ICI 182,780 alone were attributed to effects other than its well-known anti-estrogenic activity. © 2007 Elsevier B.V. All rights reserved.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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