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Type: Artigo de evento
Title: Cgi-55 Interacts With Nuclear Proteins And Co-localizes To P80-coilin Positive-coiled Bodies In The Nucleus
Author: Lemos T.A.
Kobarg J.
Abstract: The human protein CGI-55 has been described as a chromo-helicase-DNA- binding domain protein (CHD)-S interacting protein and was also found to interact with the 3′-region of the plasminogen activator inhibitor (PAI)-1 mRNA. Here, we used CGI-55 as a "bait" in a yeast two-hybrid screen and identified eight interacting proteins: Daxx, Topoisomerase I binding RS (Topors), HPC2, UBA2, TDG, and protein inhibitor of activated STAT (signal transducer and activator of transcription) (PIAS)-1, -3, and -y These proteins are either structurally or functionally associated with promyelocytic leukemia nuclear bodies (PML-NBs), protein sumoylation, or the regulation of transcription. The interactions of CGI-55 with Daxx, Topors, PIASy and UBA2 were confirmed by in vivo co-localization experiments in HeLa cells, by using green (GFP) and red fluorescence fusion proteins. A mapping study of the CGI-55 binding site for these proteins revealed three distinct patterns of interaction. The fact that CGI-55-GFP has been localized in cytoplasm and nucleus in a dotted manner, and its interaction with proteins associated with PML-NBs, suggested that CGI-55 might be associated with nuclear bodies. Although Daxx and Topors co-localized with promyelocytic leukemia protein (PML), CGI-55 itself as well as PIASy and UBA2 showed only little co-localization with PML. However, we observed that CGI-55 localizes to the nucleolus and co-localizes with p80-coilin positive nuclear-coiled bodies. © Copyright 2006 by Humana Press Inc. All rights of any nature whatsoever reserved.
Rights: fechado
Identifier DOI: 10.1385/CBB:44:3:463
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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