Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/103670
Type: Artigo de periódico
Title: Identification Of Novel Candidate Genes For Globin Regulation In Erythroid Cells Containing Large Deletions Of The Human β-globin Gene Cluster
Author: de Andrade T.G.
Peterson K.R.
Cunha A.F.
Moreira L.S.
Fattori A.
Saad S.T.O.
Costa F.F.
Abstract: The genetic mechanisms underlying the continued expression of the γ-globin genes during the adult stage in deletional hereditary persistence of fetal hemoglobin (HPFH) and δβ-thalassemias are not completely understood. Herein, we investigated the possible involvement of transcription factors, using the suppression subtractive hybridization (SSH) method as an initial screen to identify differentially expressed transcripts in reticulocytes from a normal and a HPFH-2 subject. Some of the detectable transcripts may participate in globin gene regulation. Quantitative real-time PCR (qRT-PCR) experiments confirmed the downregulation of ZHX2, a transcriptional repressor, in two HPFH-2 subjects and in a carrier of the Sicilian δβ-thalassemia trait. The chromatin remodeling factors ARID1B and TSPYL1 had a very similar pattern of expression with an incremental increase in HPFH and decreased expression in δβ-thalassemia. These differences suggest a mechanism to explain the heterocellular and pancellular distribution of fetal hemoglobin in δβ-thalassemia and deletional HPFH, respectively. Interestingly, α-globin mRNA levels were decreased, similar to β-globin in all reticulocyte samples analyzed. © 2006 Elsevier Inc. All rights reserved.
Editor: 
Rights: fechado
Identifier DOI: 10.1016/j.bcmd.2006.07.003
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-33748785158&partnerID=40&md5=abfbbef4d5c66e783a2f21bbbd3a7447
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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