Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/1032
Type: Artigo de periódico
Title: Biological effects of anionic meso-tetrakis (para-sulfonatophenyl) porphyrins modulated by the metal center. Studies in rat liver mitochondria
Author: PESSOTO, Felipe Samuel
INADA, Natalia Mayumi
NEPOMUCENO, Maria de Fatima
RUGGIERO, Ana Celia
NASCIMENTO, Otaciro R.
VERCESI, Anibal E.
NANTES, Iseli L.
Abstract: In this paper, we present a study about the influence of the porphyrin metal center and mesa ligands on the biological effects of meso-tetrakis porphyrins. Different from the cationic meso-tetrakis 4-N-methyl pyridinium (Mn(III)TMPyP), the anionic Mn(III) meso-tetrakis (para-sulfonatophenyl) porphyrin (Mn(III)TPPS4) exhibited no protector effect against Fe(citrate)-induced lipid oxidation. Mn(III)TPPS4 did not protect mitochondria against endogenous hydrogen peroxide and only delayed the swelling caused by tert-BuOOH and Ca(2+). Fe(III)TPPS4 exacerbated the effect of the tert-BuOOH, and both porphyrins did not significantly affect Fe(II)citrate-induced swelling. Consistently, Fe(III)TPPS4 predominantly promotes the homolytic cleavage of peroxides and exhibits catalytic efficiency ten-fold higher than Mn(III)TPPS4. For Mn(III)TPPS4, the microenvironment of rat liver mitochondria favors the heterolytic cleavage of peroxides and increases the catalytic efficiency of the manganese porphyrin due to the availability of axial ligands for the metal center and reducing agents such as glutathione (GSH) and proteins necessary for Compound II (oxomanganese IV) recycling to the initial Mn(III) form. The use of thiol reducing agents for the recycling of Mn(III)TPPS4 leads to GSH depletion and protein oxidation and consequent damages in the organelle. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
Subject: Meso-tetrakis porphyrins
Oxidative stress
Peroxidases
Catalase
EPR
Rat liver mitochondria
Country: Irlanda
Editor: ELSEVIER IRELAND LTD
Citation: CHEMICO-BIOLOGICAL INTERACTIONS, v.181, n.3, p.400-408, 2009
Rights: fechado
Identifier DOI: 10.1016/j.cbi.2009.07.012
Address: http://dx.doi.org/10.1016/j.cbi.2009.07.012
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Date Issue: 2009
Appears in Collections:FCM - Artigos e Materiais de Revistas Científicas

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