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Type: Artigo de evento
Title: The Effects Of Copper Ions On The Synthesis Of Periplasmic And Membrane Proteins In Acidithiobacillus Ferrooxidans As Analyzed By Sds-page And 2d-page
Author: Felicio A.P.
Garcia Jr. O.
Bertolini M.C.
Ottoboni L.M.M.
Novo M.T.M.
Abstract: The effects of 200 mM copper ions on the synthesis of membrane and periplasmic proteins were investigated in iron-grown cells of Acidithiobacillus ferrooxidans (At. ferrooxidans). Total membrane protein profiles of cells grown in the absence of copper ions (unadapted cells) and in the presence of copper ions (copper-adapted cells) were compared by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Crude preparations of outer membrane and periplasmic proteins were analyzed by SDS-PAGE. The synthesis of proteins was diminished or increased in the presence of copper ions. Low molecular weight proteins (<14 kDa) were significantly repressed by copper. These proteins are probably acidic proteins located in the outer membrane. An over-expression of a periplasmic protein of about 17 kDa was detected in the copper-adapted cells and was assumed to be rusticyanin, a 16.5-kDa periplasmic copper protein present in At. ferrooxidans cells and involved in the electron-transport chain of the iron oxidation pathway. To our knowledge, this is the first report of a possible involvement of the rusticyanin and outer membrane proteins in the mechanism of copper resistance in At. ferrooxidans. © 2003 Elsevier B.V. All rights reserved.
Rights: fechado
Identifier DOI: 10.1016/S0304-386X(03)00153-1
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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