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Type: Artigo de periódico
Title: Purification And Characterization Of An N-acetylglucosamine-binding Lectin From Koelreuteria Paniculata Seeds And Its Effect On The Larval Development Of Callosobruchus Maculatus (coleoptera: Bruchidae) And Anagasta Kuehniella (lepidoptera: Pyralidae)
Author: Macedo M.L.R.
Damico D.C.S.
Freire M.D.G.M.
Toyama M.H.
Marangoni S.
Novello J.C.
Abstract: This study describes the purification of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effects on the larval development of Callobruchus maculatus and Anagasta kuehniella. The lectin (KpLec) was characterized and isolated by gel filtration, affinity column, and reverse phase chromatography. SDS-PAGE indicated that this lectin is a dimer composed of subunits of 22 and 44 kDa. The N terminus exhibited 40% similarity with Urtiga dioica agglutinin. KpLec was tested for anti-insect activity against C. maculatus and A. kuehniella. With regard to C. maculatus, an artificial diet containing 0.7 and 1% KpLec produced LD 50 and ED 50 value, respectively. However, for A. kuenhiella, an artificial diet containing 0.65% KpLec produced an LD 50, whereas 0.2% KpLec produced an ED 50. The transformation of genes coding for this lectin could be useful in the development of insect resistance in important agricultural crops.
Rights: fechado
Identifier DOI: 10.1021/jf034013i
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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