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Type: Artigo de periódico
Title: Effect Of Atorvastatin On Apoe And Apoc-i Synthesis And Secretion By Thp-1 Macrophages
Author: Castilho L.N.
Chamberland A.
Boulet L.
Davignon J.
Cohn J.S.
Bernier L.
Abstract: Apolipoprotein (apo) E and C-I are plasma apolipoproteins that have been implicated in the etiology of atherosclerosis and obesity, respectively. Both proteins are synthesized and secreted by macrophages, though pharmacological regulation of their production is poorly understood. The authors compared the effect of 2 HMG-CoA reductase inhibitors, atorvastatin and cerivastatin, on the synthesis and secretion of apoE and apoC-I by THP-1 macrophages. Atorvastatin reduced medium apoE and cellular apoE mRNA of PMA-activated THP-1 cells in a dose-dependent manner (-24% and -22%, respectively, at 1-μmol/L, P < 0.01). ApoC-I in the medium was also reduced by atorvastatin in a dose-dependent manner, though to a lesser extent (-15% at 1-μmol/L, P < 0.05). Cerivastatin similarly reduced medium apoE (-20% at 1-μmol/L, P < 0.05) and cellular apoE mRNA (-31% at 1-μmol/L, P < 0.05), and significantly lowered cellular apoC-I mRNA (-15%, P < 0.05), but not apoC-I in the medium. In experiments with THP-1 macrophages loaded with cholesterol (ie, 24-hour incubation with acetyl-LDL), atorvastatin and cerivastatin (1-μmol/L) significantly (P < 0.05) reduced both medium apoE (-30% and -25%, respectively) and cellular apoE mRNA (-25% and -17%, respectively). A lower and less consistent effect was observed on medium apoC-I (-6% and -18%, respectively) and cellular apoC-I mRNA (-13% and -19%, respectively). These data demonstrate that statins have the capacity to reduce the synthesis and secretion of both apoE and apoC-I in THP-1 macrophages loaded or unloaded with cholesterol.
Rights: fechado
Identifier DOI: 10.1097/00005344-200308000-00015
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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