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Type: Artigo de periódico
Title: Inflammatory Oedema Induced By Phospholipases A2 Isolated From Crotalus Durissus Sp. In The Rat Dorsal Skin: A Role For Mast Cells And Sensory C-fibers
Author: Camara P.R.S.
Esquisatto L.C.M.
Camargo E.A.
Ribela M.T.C.P.
Toyama M.H.
Marangoni S.
De Nucci G.
Antunes E.
Abstract: The ability of the phospholipases A2 (PLA2s) from Crotalus durissus cascavella, Crotalus durissus collilineatus and Crotalus durissus terrificus venoms and crotapotin to increase the vascular permeability in the rat skin as well as the contribution of both mast cells and sensory C-fibers have been investigated in this study. Vascular permeability was measured as the plasma extravascular accumulation at skin sites of intravenously injected 125I-human serum albumin. Intradermal injection of crotalic PLA2s (0.05-0.5 μg/site) in the rat skin resulted in dose-dependent increase in plasma extravascular whereas crotapotin (1 μg/site) failed to affect this response. Co-injection of crotapotin (1 μg/site) did not modify the increased vascular permeability induced by the PLA2s (0.05-0.5 μg/site). Previous treatment (30 min) of the animals with cyproheptadine (2 mg/kg, i.p.) markedly reduced PLA2 (0.5 μg/site)-induced oedema. In rats treated neonatally with capsaicin to deplete neuropeptides, the plasma extravasation induced by all PLA2s (0.5 μg/site) was also significantly reduced. Similarly, the tachykinin NK1 receptor antagonist SR140333 (1nmol/site) significantly reduced the PLA2-induced oedema. In addition, the combination of SR140333 with cyproheptadine further reduced the increased plasma extravasation by PLA2 from C. d. cascavella venom, but not by PLA2 from C. d. terrificus and C. d. collilineatus venoms. Our results suggest that increase in skin vascular permeability by crotalic PLA2s is mediated by activation of sensory C-fibers culminating in the release of substance P, as well as by activation of mast cells which in turn release amines such as histamine and serotonin. © 2003 Elsevier Science Ltd. All rights reserved.
Rights: fechado
Identifier DOI: 10.1016/S0041-0101(03)00037-0
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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