Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/101813
Type: Artigo de periódico
Title: Isolation And Preliminary Enzymatic Characterization Of A Novel Pla 2 From Crotalus Durissus Collilineatus Venom
Author: Ponce-Soto L.A.
Toyama M.H.
Hyslop S.
Novello J.C.
Marangoni S.
Abstract: A crotoxin homolog was purified from the Crotalus durissus collilineatus venom using molecular exclusion and reverse-phase HPLC. This crotoxin contained one PLA2 (Cdcolli III F6) and four crotapotin isoforms, whereas crotoxin from Crotalus durissus terrificus venom had three PLA2 isoforms and two crotapotin isoforms. SDS-PAGE showed that the C. d. collilineatus PLA2 and crotapotin had relative molecular mass of 15 and 9 kDa, respectively. Neither the PLA2 (Cdcolli III F6) nor the crotapotins (Cdcolli III F3 and F4) had any neurotoxicity in mouse phrenic nerve-diaphragm preparations when tested alone. However, when PLA2 and crotapotin were coincubated before testing, the neurotoxicity was restored to a level similar to test in the venom in native crotoxin. The two crotapotins (Cdcolli III F3 and F4) differed in their ability to inhibit PLA2 activity, perhaps because of variations in their affinities for this enzyme. Cdcolli III F6 showed allosteric enzymatic behavior, with maximal activity at pH 8.3 and 36°C. Full PLA2 activity required the presence of a low Ca2+ concentration and was inhibited by Cu2+ and Zn 2+ and by Cu2+ and Mg2+ in the presence and absence of Ca2+, respectively. These results indicate that crotoxin from C. d. collineatus venom is very similar enzymatically to crotoxin from C. d. terrificus. © 2002 Plenum Publishing Corporation.
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Rights: fechado
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Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-52649174864&partnerID=40&md5=f887201a2d4a06a1a1a76f97578ddec9
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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