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Type: Artigo de evento
Title: Expression Of A Thioredoxin Peroxidase In Insulin-producing Cells
Author: Boschero A.C.
Stoppiglia L.F.
Collares-Buzato C.B.
Bosqueiro J.R.
Delghingaro-Augusto V.
Leite A.
Carvalho C.P.F.
Netto L.E.S.
Carneiro E.M.
Abstract: The presence of thioredoxin peroxidase (TPx), also known as thiol specific antioxidant (TSA), was investigated in neonatal and adult rat islets, and in the β-cell line HIT-T15. Western blotting of extracts from neonatal and adult pancreatic islets and from the tumoral cell line HIT-T15 revealed the presence of a 25 kDa protein that comigrated with purified yeast TPx. Endocrine pancreatic TPx accounted for approximately 0.01% of the total protein content. Treatment with H 2O 2 for 3 h increased the expression of TPx in HIT-T15 cells. The distribution of TPx throughout the islet cells was confirmed by immunocytochemistry. Since pancreatic β-cells possess a weak antioxidant enzyme defense system, especially with regard to hydrogen peroxidase-decomposing enzymes, the presence of a TPx analog in islets suggests that this enzyme may play a role in protecting pancreatic cells against reactive oxygen species.
Rights: fechado
Identifier DOI: 
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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