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Type: Artigo de periódico
Title: Comparative Study Of The Obtention Of Crude And Semi-purified Lipase Extracts From Rice Bran [estudio Comparative De La Obtención De Extractos Crudos Y Semipurificados De Lipasa De Salvado De Arroz]
Author: Bella Cruz A.
Barrera-Arellano D.
Abstract: Crude lipase extracts from defatted rice bran were obtained using three different extraction solutions: 1 - CaCl2 0.01M; 2 - Sacarose 0.6M, KCl 10mM, MgCl2 1mM, Ditiotreitol 2mM and 3 - Sacarose 0.4M, Ditiotreitol 2mM. Factors (variables) studied were pH (6.0, 7.0, 8.0) and extraction time (1h, 2h, 3h). Extraction temperature was 4 °C. The extracts obtained presented different lipase activities, and the crude extract obtained with CaCl2 0.01M was the one with the highest specific activity (12.1mU/mg). Partial purification of this crude extract with acetone and ammonium sulfate resulted in fractions with specific activities of 50.7mU/mg and 63.5mU/mg, respectively.
Rights: aberto
Identifier DOI: 
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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