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Type: Artigo de periódico
Title: Automated Measurement Of Mouse Freezing Behavior And Its Use For Quantitative Trait Locus Analysis Of Contextual Fear Conditioning In (balb/cj X C57bl/6j)f2 Mice
Author: Valentinuzzi V.S.
Kolker D.E.
Vitaterna M.H.
Shimomura K.
Whiteley A.
Low-Zeddies S.
Turek F.W.
Ferrari E.A.M.
Paylor R.
Takahashi J.S.
Abstract: The most commonly measured mouse behavior in fear conditioning tests is freezing. A technical limitation, particularly for genetic studies, is the method of direct observation used for quantifying this response, with the potential for bias or inconsistencies. We report the use of a computerized method based on latency between photobeam interruption measures as a reliable scoring criterion in mice. The different computer measures obtained during contextual fear conditioning tests showed high correlations with hand-scored freezing; r values ranged from 0.87 to 0.94. Previously reported strain differences between C57BL/6J and DBA/2J in context-dependent fear conditioning were also detected by the computer-based system. In addition, the use of computer-scored freezing of 199 (BALB/cJ x C57BL/6J)F2 mice enabled us to detect a suggestive gender-dependent chromosomal locus for contextual fear conditioning on distal chromosome 8 by QTL analysis. Automation of freeze scoring would significantly increase efficiency and reliability of this learning and memory test.
Rights: fechado
Identifier DOI: 
Date Issue: 1998
Appears in Collections:Unicamp - Artigos e Outros Documentos

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