Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/100549
Type: Artigo de evento
Title: Expanded-bed Adsorption Utilizing Ion-exchange Resin To Purify Extracellular β-galactosidase
Author: Pereira J.A.M.
De Rosa P.T.V.E.
Pastore G.M.
Santana C.C.
Abstract: The application of expanded-bed ion-exchange resins allows the elimination of intermediary particulate separation steps like filtration or centrifugation prior to adsorption steps in enzyme-purification processes from crude fermentation broths. This work is concerned with the experimental evaluation data of a process related to the adsorption of an extracellular β-galactosidase from the fungi Scopulariopsis. The protein recovery in the ion-exchange resin Accell Plus QMA(TM) was accomplished using a continuous- monitoring method. The direct adsorption step was followed by a elution step with concentrated NaCl solutions aiming to improve the enzyme-specific activity. Experimental data for fixed and expanded bed were compared.
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Rights: fechado
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Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-0031858295&partnerID=40&md5=f38e3a0bb36817aecaf7a38f32215879
Date Issue: 1998
Appears in Collections:Unicamp - Artigos e Outros Documentos

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