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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleAn Unusual Presynaptic Action Of Bothrops Insularis Snake Venom Mediated By Phospholipase A2 Fractionpt_BR
dc.contributor.authorCogo J.C.pt_BR
dc.contributor.authorPrado-Franceschi J.pt_BR
dc.contributor.authorGiglio J.R.pt_BR
dc.contributor.authorCorrado A.P.pt_BR
dc.contributor.authorCruz-Hofling M.A.pt_BR
dc.contributor.authorDonato J.L.pt_BR
dc.contributor.authorLeite G.B.pt_BR
dc.contributor.authorRodrigues-Simioni L.pt_BR
unicamp.authorCogo, J.C., Department of Physiology, Institute of Biology, UNICAMP, Campinas, S.P., Brazilpt_BR
unicamp.authorPrado-Franceschi, J., Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, Campinas, S.P., Brazilpt_BR
unicamp.authorCruz-Höfling, M.A., Dept. of Histology and Embryology, Institute of Biology, UNICAMP, Campinas, S.P., Brazilpt_BR
unicamp.authorDonato, J.L., Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, Campinas, S.P., Brazilpt_BR
unicamp.authorLeite, G.B., Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, Campinas, S.P., Brazilpt_BR
unicamp.authorRodrigues-Simioni, L., Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, Campinas, S.P., Brazilpt_BR
unicamp.author.externalGiglio, J.R., Department of Biochemistry, Faculty of Medicine, U.S.P., Ribeirao Preto, S.P., Brazilpt
unicamp.author.externalCorrado, A.P., Department of Pharmacology, Faculty of Medicine, U.S.P., Ribeirao Preto, S.P., Brazilpt
dc.description.abstractA phospholipase A2-contraining fraction was isolated from the venom of Bothrops insularis by a combination of gel filtration on Sephadex G-150 and ion exchange chromatography on DEAE-Sephadex. Peak IV of the latter chromatography containing all of the phospholipase A2 (PLA2) activity, was assayed on isolated neuromuscular preparations. In the mouse phrenic nerve- diaphragm incubated in Tyrode at 37°C, the PLA2 fraction produced an initial increase in the twitch tension and in the frequency of the mepps, followed by a dose-dependent, irreversible blockade. The replacement of 1.8 mM Ca2+ by 4 mM Sr2+ inhibited the neuromuscular blocking effect of the fraction. In the chick biventer cervicis preparation incubated with Krebs solution at 37°C, the PLA2 fraction induced blockade but did not affect the response to acetylcholine and K+, excluding the involvement of post-synaptic and direct muscular effects. A low temperature (18-22°C) incubation prevented the neuromuscular effect from developing. These results suggest that the PLA2-containing fraction acts predominantly at presynaptic sites at the neuromuscular junction. This fraction also accounts for most Of the pharmacological effects of the crude venom.en
dc.relation.ispartofToxiconpt_BR
dc.date.issued1998pt_BR
dc.identifier.citationToxicon. , v. 36, n. 10, p. 1323 - 1332, 1998.pt_BR
dc.language.isoenpt_BR
dc.description.volume36pt_BR
dc.description.issuenumber10pt_BR
dc.description.firstpage1323pt_BR
dc.description.lastpage1332pt_BR
dc.rightsfechadopt_BR
dc.sourceScopuspt_BR
dc.identifier.issn410101pt_BR
dc.identifier.doi10.1016/S0041-0101(97)00174-8pt_BR
dc.identifier.urlhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0031859758&partnerID=40&md5=60268f94887e47b8fc46891558861504pt_BR
dc.date.available2015-06-30T15:04:18Z
dc.date.available2015-11-26T14:57:43Z-
dc.date.accessioned2015-06-30T15:04:18Z
dc.date.accessioned2015-11-26T14:57:43Z-
dc.description.provenanceMade available in DSpace on 2015-06-30T15:04:18Z (GMT). No. of bitstreams: 1 2-s2.0-0031859758.pdf: 503630 bytes, checksum: 74c4a43f1bd7e2d82caf8ab1072ea458 (MD5) Previous issue date: 1998en
dc.description.provenanceMade available in DSpace on 2015-11-26T14:57:43Z (GMT). No. of bitstreams: 2 2-s2.0-0031859758.pdf: 503630 bytes, checksum: 74c4a43f1bd7e2d82caf8ab1072ea458 (MD5) 2-s2.0-0031859758.pdf.txt: 22624 bytes, checksum: e21b3d159753cf4d2be21e1d65152c6e (MD5) Previous issue date: 1998en
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/100439
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/100439-
dc.identifier.idScopus2-s2.0-0031859758pt_BR
dc.description.referenceBreithaupt, H., Enzymatic characteristics of Crotalus PLA2 and crotoxin complex (1976) Toxicon, 14, p. 221pt_BR
dc.description.referenceBulbring, E., Observation on the isolated phrenic nerve diaphragm preparation on the rat (1946) Brit. J. Pharmacol., 1, p. 38pt_BR
dc.description.referenceCogo, J.C., (1995) Estudo Da Fração Fosfolipasica A2 Isolada Do Veneno de Bothrops Insularis Na Junção Neuromuscular, , Doctoral thesis, Universidade Estadual de Campinas, S.P., Brasilpt_BR
dc.description.referenceCogo, J.C., Prado-Franceschi, J., Cruz-Hofling, M.A., Corrado, A.P., Rodrigues Simioni, L., Effects of Bothrops insularis venom on the mouse and chick nerve-muscle preparation (1993) Toxicon, 31, pp. 1237-1247pt_BR
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dc.description.referenceHo, C.L., Tsai, I.H., Lee, C.Y., The role of enzyme activity and charge properties on the presynaptic neurotoxicity and the contracture-inducing activity of snake venom phospholipase A2 (1986) Toxicon, 24, p. 337pt_BR
dc.description.referenceHumel, B.C.W., A modified spectrophotometric determination of chymotrypsin and thrombin (1959) Can. J. Biochem., 37, p. 1393pt_BR
dc.description.referenceItzhaki, R.F., Gill, M.D., A micro-biuret method for estimating proteins (1964) Anal. Biochem., 9, p. 401pt_BR
dc.description.referenceKunitz, M., Crystalline soybean trypsin inhibitor. II. General properties (1947) J. Gen. Physiol., 30, p. 291pt_BR
dc.description.referenceLobo-Araújo, A., Radvany, F., Determination of phospholipase A2 activity by a colorimetric assay using a pH indicator (1987) Toxicon, 25, pp. 1181-1188pt_BR
dc.description.referenceMandelbaum, F.R., Reichl, A.P., Assakura, M.T., Isolation and characterization of a proteolytic enzyme from the venom of the snake Bothrops jararaca (jararaca) (1982) Toxicon, 20, pp. 955-972pt_BR
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