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Type: Artigo de periódico
Title: Use Of Chemically Modified Silica With β-diketoamine Groups For Separation Of α-lactoalbumin From Bovine Milk Whey By Affinity Chromatography
Author: Gambero A.
Kubota L.T.
Gushikem Y.
Airoldi C.
Granjeiro J.M.
Taga E.M.
Alcantara E.F.C.
Abstract: Silica gel surface was chemically modified with β-diketoamine groups by reacting the silanol from the silica surface with 3-aminopropyl-triethoxysilane and 3-bromopentanedione. With this material, copper ions were adsorbed from aqueous solutions. The chemical analysis of the silica-gel-immobilized acetylacetone provided a quantity of 0.67 mmol g-1 of organic groups attached to the support and 0.63 mmol g-1 of copper. This material was used as a stationary phase in IMAC (immobilized metal affinity chromatography), to separate α-lactoalbumin from bovine milk whey. The results showed an efficient separation in the chromatographic column. The possibility of reutilization of the stationary phase was also investigated.
Rights: fechado
Identifier DOI: 10.1006/jcis.1996.4566
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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