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Type: Artigo de periódico
Title: Extraction Of Recombinant Cytochrome B5 From Disrupted Escherichia Coli Cells With An Aqueous Two-phase System In A Continuous Perforated Rotating Disc Contactor
Author: Porto A.L.F.
Lima-Filho J.L.
Aires-Barros M.R.
Cabral J.M.S.
Tambourgi E.B.
Abstract: A continuous perforated rotating disc contactor was used to extract recombinant cytochrome b5 from disrupted Escherichia coli cells with an aqueous two-phase system. About 4.9 mg (44%) total protein and 5 mg (75%) cytochrome b5 were recovered to the salt-rich phase, using PEG (18% w/w)-KH(2PO4/K2HPO4) (16% w/w) salt system. The dispersed phase (PEG) hold up increased with increasing rotor velocity. The mass transfer coefficient and separation efficiency for cytochrome, normalized for the PEG hold up, depended upon the disc's rotation speed.
Rights: fechado
Identifier DOI: 
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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