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|Type:||Artigo de periódico|
|Title:||Large-scale Production And Purification Of Recombinant Protein From An Insect Cell/baculovirus System In Erlenmeyer Flasks: Application To The Chicken Poly(adp-ribose) Polymerase Catalytic Domain|
|Abstract:||A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 × 109 infected cells in three liters of culture.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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