Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/100218
Type: Artigo de periódico
Title: Cdna-derived Amino-acid Sequence Of A Land Turtle (geochelone Carbonaria) β-chain Hemoglobin
Author: Bordin S.
Meza A.N.
Saad S.T.O.
Ogo S.H.
Costa F.F.
Abstract: The cDNA sequence encoding the turtle Geochelone carbonaria β-chain was determinated. The isolation of hemoglobin mRNA was based on degenerate primers' PCR in combination with 5'- and 3'-RACE protocol. The full length cDNA is 615 bp with the ATG start codon at position 53 and TGA stop codon at position 495; The AATAAA polyadenylation signal is found at position 599. The deduced polypeptyde contains 146 amino-acid residues. The predicted amino acid sequence shares 83% identity with the β-globin of a related specie, the aquatic turtle C. p. belli. Otherwise, identity is higher when compared with chicken β-Hb (80%) than with other reptilian orders (Squamata, 69%, and Crocodilia, 61%). Compared with human HbA, there is 67% identity, and at least three amino acid substitutions could be of some functional significance (Glu43β→Ser, His116β→Thr and His143β→Leu). To our knowledge this represents the first cDNA sequence of a reptile globin gene described.
Editor: 
Rights: fechado
Identifier DOI: 
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-0031398384&partnerID=40&md5=7fa3ba9235281920b443448854bdfa49
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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