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Type: Artigo de periódico
Title: Inhibition Of Succinic Dehydrogenase And F0f1-atp Synthase By 4,4'-diisothiocyanatostilbene-2,2'-disulfonic Acid (dids)
Author: Bernardes C.F.
Meyer-Fernandes J.R.
Martins O.B.
Vercesi A.E.
Abstract: This study shows that incubation of rat liver mitochondria in the presence of the thiol/amino reagent 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) is followed by inhibition of both succinate supported respiration and oxidative phosphorylation. Half-maximal inhibition of succinic dehydrogenase activity and succinate oxidation by mitochondria was attained at 55.3 and 60.8 μM DIDS, respectively. DIDS did inhibit the net ATP synthesis and ATP mutually implies [32P]Pi exchange reaction catalyzed by submitochondrial particles in a dose-dependent manner (K(i) = 31.7 μM and K(i) = 32.7 μM), respectively. The hydrolytic activities of uncoupled heart submitochondrial particles and purified F1-ATPase were also inhibited 50% by 31.9 and 20.9 μM DIDS, respectively.
Rights: fechado
Identifier DOI: 
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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