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Type: Artigo de periódico
Title: Inhibition Of Bovine Kidney Low Molecular Weight Acid Phosphatase By Uric Acid
Author: Aoyama H.
Abstract: Uric acid plays an important role in the purinic nucleotides metabolism. In this work we studied the effect of this compound on the bovine kidney low molecular weight acid phosphatase activity. Uric acid concentration that inhibited 50% of activity was 1.0, 0.4, 1.3, and 0.2 mM, respectively for pnitrophenyl phosphate (pNPP), flavine mononueleotide,/%naphtyl phosphate and tyrosine phosphate as substrates. For the pNPP-directed hydrolysis the inhibitory effect was fully reversible with a Ki value of 0.2 mM. The inhibition by uric acid shifted the optimum pH from 5.0 to 6.5; the inhibition rate was higher at pH 5.0 than at pH 6.5. The enzyme-uric acid interaction was prevented by 10 mM of inorganic phosphate but not by thiol compounds. Other Sit-dependent acid phosphatases were not inhibited by uric acid. Our results suggest that the inhibition by the physiological compound uric acid does not involve SH residues of the enzyme. Financial Support: FAPESP, CAPES, CNPq, FAEP-UNICAMP.
Rights: fechado
Identifier DOI: 
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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