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Type: Artigo de periódico
Title: Promoter Tissue Specific Activity And Ethylene Control Of The Gene Coding For The Maize Hydroxyproline-rich Glycoprotein In Maize Cells Transformed By Particle Bombardment
Author: Menossi M.
Martinez-Izquierdo J.A.
Puigdomenech P.
Abstract: The translational construct, containing 719 bp of promoter and 5'- untranslated region and the first 16 bp of coding region, of the maize gene Hrgp encoding a hydroxyproline-rich glycoprotein fused to a glucuronidase reporter cassette, has been tested for activity in different maize tissues by microprojectile bombardment. The promoter has been found to be very active in the tissues of the plant, such as meristems or young shoots, with high cell wall formation activity where a high expression has also been shown for the endogenous gene. The promoter was also shown to be very active in cell types with a protection role such as in pericarp or styles and in cell types where the reinforcement of the cell wall is needed, as styles, auricles and cortical cells in the root tip. The promoter activity is developmentally regulated in the endosperm, being highest simultaneously with active cell division at the early-mid stages of development. In the presence of ethylene, the promoter shows an increased activity in accordance with the increment of mRNA accumulation observed in the plant upon ethylene treatment. It is concluded that the promoter fragment starting at -719 bp (numbering related to the ATG) of the Hrgp gene keeps the essential cis-DNA elements necessary for spatial, temporal and hormonal gene expression in maize.
Rights: fechado
Identifier DOI: 10.1016/S0168-9452(97)00066-6
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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