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Type: Artigo de periódico
Title: Genes Coding For Shiga-like Toxins In Bovine Verotoxin-producing Escherichia Coli (vtec) Strains Belonging To Different O:k:h Serotypes
Author: Blanco M.
Blanco J.
Blanco J.E.
Gonzalez E.A.
Gomes T.A.T.
Zerbini L.F.
Yano T.
de Castro A.F.P.
Abstract: Forty-six verotoxin-producing Escherichia coli (VTEC) strains isolated from diarrhoeic and healthy calves in Spain were examined for DNA sequences homologous to genes for verotoxins (VT1 and VT2) and enterotoxins (LT-I, LT-II, STaH, STaP and STb). Hybridisation showed that 26 (57%) of VTEC strains carried VT1 genes, 13 (28%) possessed VT2 genes, and 7 (15%) carried both VT1 and VT2 genes. No VTEC strains hybridised with DNA probes for enterotoxins. A correlation was found between the serotype and type of VT produced. Thus, all strains of serotypes O26:K-:H11 (13 strains), O103:K-:H2 (3 strains) and O128:K?:H- (4 strains) hybridised with the VT1 probe only, whereas all strains of serotypes O4:K-:H4 (3 strains) and O113:K-:H21 (4 strains) were positive with the VT2 probe only. By contrast, O81:K?:H28 (2 strains) and O157:K-:H- (2 strains) strains hybridised with both VT1 and VT2 probes. One strain of serotype O157:K-:H7 was VT2 positive. © 1994.
Citation: Veterinary Microbiology. , v. 42, n. 2-3, p. 105 - 110, 1994.
Rights: fechado
Identifier DOI: 10.1016/0378-1135(94)90010-8
Date Issue: 1994
Appears in Collections:Unicamp - Artigos e Outros Documentos

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