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Type: Artigo de periódico
Artigo de periódico
Title: Dehydrocrotonin And Its β-cyclodextrin Complex: Cytotoxicity In V79 Fibroblasts And Rat Cultured Hepatocytes
Author: Correa D.H.A.
Melo P.S.
De Carvalho C.A.A.
De Azevedo M.B.M.
Duran N.
Haun M.
Abstract: Trans-dehydrocrotonin has antiulcerogenic and antitumor activities. A complex of β-cyclodextrin with dehydrocrotonin was developed to improve the delivery of dehydrocrotonin. Complex in solid state was evaluated using X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA) and scanning electron microscopy (SEM). X-ray diffraction and scanning electron microscopy studies showed that dehydrocrotonin exists in a semicrystalline state in the complexed form with β-cyclodextrin. Differential scanning calorimetry studies showed the existence of a complex of dehydrocrotonin with β-cyclodextrin. The thermal gravimetric analysis studies confirmed the differential scanning calorimetry results of the complex. Free dehydrocrotonin and the dehydrocrotonin/β-cyclodextrin inclusion complex were assayed in freshly isolated rat hepatocytes and in V79 cells. Cytotoxicity was determined using nucleic acid content, methylthiazoletetrazolium (MTT) reduction and neutral red uptake assays. In all assays, there was a large reduction (3.5-16.1-fold) in the cytotoxicity of dehydrocrotonin in hepatocytes when complexed with β-cyclodextrin, whereas for V79 cells the decrease in cytotoxicity was 1.7- and 1.87-fold for MTT reduction and nucleic acid content assays, respectively. The lower cytotoxicity of the dehydrocrotonin/β-cyclodextrin complex compared to free dehydrocrotonin in rat hepatocytes and V79 cells suggests that such a complex may be useful for the administration of dehydrocrotonin in vivo. © 2005 Elsevier B.V. All rights reserved.
Citation: European Journal Of Pharmacology. , v. 510, n. 1-2, p. 17 - 24, 2005.
Rights: fechado
Identifier DOI: 10.1016/j.ejphar.2005.01.016
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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