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Type: Artigo de evento
Title: Evidence Of Chemotaxis By Quantitative Measurement Of The Force Vectors Of Trypanossoma Cruzi In The Vicinity Of The Rhodnius Prolixus Midgut Wall Cell
Author: De Thomaz A.A.
Almeida D.B.
Fontes A.
Stahl C.V.
Santos-Mallet J.R.
Gomes S.A.O.
Feder D.
Cesar C.L.
Abstract: In this work we used a methodology to study chemotaxis of Trypanossoma cruzi (T. Cruzi) in real time using an Optical Tweezers system. Trapped beads were used as a force transducer for measuring forces of the same order of magnitude as typical forces induced by flagellar motion. Optical Tweezers allowed real time measurements of the force vectors, strength and direction, of living parasites under chemical or other kinds of gradients. This seems to be the ideal tool to perform observations of taxis response of cells and microorganisms with high sensitivity to capture instantaneous responses to a given stimulus. We applied this methodology to investigate the T. cruzi under distinct situations: the parasite alone and in the presence of its insect-vector Rhodnius prolixus (R. prolixus). © 2009 SPIE.
Citation: Proceedings Of Spie - The International Society For Optical Engineering. , v. 7400, n. , p. - , 2009.
Rights: aberto
Identifier DOI: 10.1117/12.826314
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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