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Type: Artigo de periódico
Title: LC-APCI-MS-MS methodology for determination of glybenclamide in human plasma
Author: Moura, MRL
de Nucci, G
Rath, S
Reyes, FGR
Abstract: A liquid chromatographic/atmospheric pressure chemical ionization tandem mass spectrometric method (LC-APCI-MS-MS) for the determination of glybenclamide in human plasma is described. Glypizide, an analogue of glybenclamide, was used as internal standard. The analyte was extracted from plasma with diethyl ether/dichloromethane (70:30 v/v). The chromatography uses C-18 and 0.01 mol L-1 acetic acid/acetonitrile (20:80 v/v) as stationary and mobile phase, respectively. Quantitation was preformed by using multiple reaction monitoring (MRM) of the precursor ion (m/z 494.2-->368.8) and the related product ion (m/z 446.0-->347.3) using the internal standard method. The analytical curve was linear in the range 1-300 ng mL(-1), and for a 400-muL sample of human plasma, the limit of determination of the method was 1 ng mL(-1). The coefficients of variation of the method for intra-assay (within-run precision) and inter-assay (between-run precision) were less than 10%. The method was shown to be suitable for pharmacokinetic studies.
Subject: glybenclamide
mass spectrometry
Country: Alemanha
Editor: Springer-verlag Heidelberg
Citation: Analytical And Bioanalytical Chemistry. Springer-verlag Heidelberg, v. 378, n. 2, n. 499, n. 503, 2004.
Rights: fechado
Identifier DOI: 10.1007/s00216-003-2248-8
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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