Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/78655
Type: Artigo de periódico
Title: Texture analysis of the epidermis based on fast Fourier transformation in Sjogren-Larsson syndrome
Author: Auada, MP
Adam, RL
Leite, NJ
Puzzi, MB
Cintra, ML
Rizzo, WB
Metze, K
Abstract: OBJECTIVE: To investigate whether image analysis of routine hematoxylin-eosin (H-E) skin sections using fast Fourier transformation (FFT) could detect structural alterations in patients with Sjogren-Larsson syndrome (SLS) diagnosed by molecular biology. STUDY DESIGN: Skin punch biopsies of 9 patients with SLS and 17 healthy volunteers were obtained. Digital images of routine histologic sections were taken, and their gray scale luminance was analyzed by FFT. The inertia values were determined for different ranges of the spatial frequencies in the vertical and horizontal direction. To get an estimation of anisotropy, we calculated the resultant vector of the designated frequency ranges. RESULTS: In the prickle cell layer, SLS patients showed more intense amplitudes in spatial structures with periods between 1.2 and 3.6 mu m in the vertical direction, which correlated in part with accentuated nuclei and nucleoli and perinucleolar halos in the H-E sections. In a linear discriminant analysis, the variables derived from the FFT images correctly discriminated 84.6% of the patients. Texture features derived from the gray level co-occurrence matrix were not able to separate the groups. CONCLUSION: Exploratory texture analysis by FFT was able to detect discrete alterations in the prickle cell layer in routine light microscopy slides of SLS patients. The structural changes identified by FFT may be related to abnormal cellular components associated with aberrant lipid metabolism.
Subject: epidermis
ichthyosis
karyometry
morphometry
nucleus
nucleolus
pathology
quantitative
Country: EUA
Editor: Sci Printers & Publ Inc
Citation: Analytical And Quantitative Cytology And Histology. Sci Printers & Publ Inc, v. 28, n. 4, n. 219, n. 227, 2006.
Rights: fechado
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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