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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleCementoblast gene expression is regulated by Porphyromonas gingivalis lipopolysaccharide partially via Toll-like receptor-4/MD-2pt_BR
dc.contributor.authorNociti, FHpt_BR
dc.contributor.authorFoster, BLpt_BR
dc.contributor.authorBarros, SPpt_BR
dc.contributor.authorDarveau, RPpt_BR
dc.contributor.authorSomerman, MJpt_BR
unicamp.author.emailsomerman@u.washington.edupt_BR
unicamp.authorUniv Washington, Sch Dent, Dept Periodont, Hlth Sci Ctr D322, Seattle, WA 98195 USA Univ Campinas, Sch Dent Piracicaba, Dept Prosthodont Periodont, Div Periodont, Piracicaba, SP, Brazil Univ Campinas, Sch Dent Piracicaba, Dept Morphol, Div Histol, Piracicaba, SP, Brazilpt_BR
dc.subjectlipopolysaccharidespt_BR
dc.subjectcementoblastspt_BR
dc.subjectcytokinespt_BR
dc.subjectPorphyromonas gingivalispt_BR
dc.subject.wosOsteoclast Differentiation Factorpt_BR
dc.subject.wosIn-vitropt_BR
dc.subject.wosParathyroid-hormonept_BR
dc.subject.wosBonept_BR
dc.subject.wosOsteoprotegerinpt_BR
dc.subject.wosCellspt_BR
dc.subject.wosInterleukin-1pt_BR
dc.subject.wosOsteopontinpt_BR
dc.subject.wosOsteoblastspt_BR
dc.subject.wosActivationpt_BR
dc.description.abstractLipopolysaccharides are potent inflammatory mediators considered to contribute to destruction of periodontal tissues. Here, we hypothesized that Porphyromonas gingivalis lipopolysaccharide (P-LPS) treatment would regulate gene expression in murine cementoblasts through Toll-like receptor 4. Real-time (RT)-PCR and Northern blot analysis indicated that P-LPS decreased expression of transcripts for osteocalcin (OCN) and receptor activator of nuclear factor kappaB ligand (RANKL). In contrast, a dose-dependent up-regulation in mRNA levels for osteopontin (OPN) and osteoprotegerin (OPG) was observed. Similarly, ELISA demonstrated decreased RANKL and increased OPG levels. A monoclonal antibody specific for mouse TLR-4/MD-2 partially neutralized the P-LPS effect on cementoblasts. These results indicate that exposure of cementoblasts to P-LPS can alter cell function by regulating markers of osteoclastic activity (e.g., RANKL/OPG), thereby potentially affecting the inflammation-associated resorption of mineralized tissues.pt
dc.relation.ispartofJournal Of Dental Researchpt_BR
dc.relation.ispartofabbreviationJ. Dent. Res.pt_BR
dc.publisher.cityAlexandriapt_BR
dc.publisher.countryEUApt_BR
dc.publisherInt Amer Assoc Dental Researchi A D R/a A D Rpt_BR
dc.date.issued2004pt_BR
dc.date.monthofcirculationAUGpt_BR
dc.identifier.citationJournal Of Dental Research. Int Amer Assoc Dental Researchi A D R/a A D R, v. 83, n. 8, n. 602, n. 607, 2004.pt_BR
dc.language.isoenpt_BR
dc.description.volume83pt_BR
dc.description.issuenumber8pt_BR
dc.description.firstpage602pt_BR
dc.description.lastpage607pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0022-0345pt_BR
dc.identifier.wosidWOS:000226598400004pt_BR
dc.date.available2014-11-20T05:42:52Z
dc.date.available2015-11-26T16:07:12Z-
dc.date.accessioned2014-11-20T05:42:52Z
dc.date.accessioned2015-11-26T16:07:12Z-
dc.description.provenanceMade available in DSpace on 2014-11-20T05:42:52Z (GMT). No. of bitstreams: 1 WOS000226598400004.pdf: 624196 bytes, checksum: f9e115b87822c6edded12db91777e149 (MD5) Previous issue date: 2004en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:07:12Z (GMT). No. of bitstreams: 2 WOS000226598400004.pdf: 624196 bytes, checksum: f9e115b87822c6edded12db91777e149 (MD5) WOS000226598400004.pdf.txt: 31126 bytes, checksum: eece6ff9bc335ed100ba750396d73a81 (MD5) Previous issue date: 2004en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78642pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/78642
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78642-
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