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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleValue of morphological criteria in diagnosing cervical HPV lesions confirmed by in situ hybridization and hybrid capture assaypt_BR
dc.contributor.authorRoteli-Martins, CMpt_BR
dc.contributor.authorAlves, VAFpt_BR
dc.contributor.authorSantos, RTMpt_BR
dc.contributor.authorMartinez, EZpt_BR
dc.contributor.authorSyrjanen, KJpt_BR
dc.contributor.authorDerchain, SFMpt_BR
unicamp.authorUNICAMP, Dept Gynecol, Campinas, Brazil Leonor Mendes de Barros Hosp, Sao Paulo, Brazil Adolfo Lutz Inst, Dept Pathol, Sao Paulo, Brazil Univ Sao Paulo, Sch Med, Sao Paulo, Brazil Univ Siena, Dept Pathol, I-53100 Siena, Italy UNICAMP, Dept Med Stat CAISM, Campinas, Brazilpt_BR
dc.subjectin situ hybridizationpt_BR
dc.subjecthybrid capturept_BR
dc.subjectmorphologypt_BR
dc.subjecthuman papillomaviruspt_BR
dc.subjectintraepithelial neoplasiapt_BR
dc.subject.wosHuman Papillomavirus Dnapt_BR
dc.subject.wosCytologic Diagnosispt_BR
dc.subject.wosIntraepithelial Neoplasiapt_BR
dc.subject.wosCondylomatous Lesionspt_BR
dc.subject.wosWomenpt_BR
dc.subject.wosAbnormalitiespt_BR
dc.subject.wosPatternspt_BR
dc.subject.wosSmearspt_BR
dc.subject.wosCancerpt_BR
dc.subject.wosRiskpt_BR
dc.description.abstractThe present study evaluated the value of morphological criteria (binucleation, multinucleation, koilocytosis, spindle koilocytes, abnormal mitosis and dyskeratosis) in the diagnosis of cervical human papillomavirus (HPV) lesions confirmed by in sitit hybridization (ISH) and hybrid capture (HC) assay. Colposcopic punch biopsies from a series of 138 women with abnormal Pap smears were examined on light microscopy and in situ hybridization (DAKO widespectrum cocktail probe) for HPV-induced morphological changes and HPV DNA, respectively. Cervical swabs were analyzed for HPV DNA of the oncogenic types using Hybrid Capture. CIN 2 and CIN 3 were found in 44 biopsies, CIN 1 in 62, and no evidence of HPV in 32 cases. HPV was detected by ISH in 51/138 (37%) cases and by HC in 66/138 (48%) lesions. With both tests, HPV DNA detection increased parallel with lesion severity, up to 70% and 59% in CIN 2/3 by HC and ISH, respectively OR 4.6 (1.7-12.1) and 10.1 (3.0-33.8). Among the histological criteria, multinucleation, binucleation and abnormal mitoses were significantly associated with HPV DNA detection. Multinucleation proved to be the strongest predictor of HPV DNA-positivity. Binucleation, abnormal mitosis, koilocytosis and spindle koilocytes were also reliable criteria of HPV lesions. Minor nuclear atypia, and "mild koilocytosis" were of no value in making this diagnosis.pt
dc.relation.ispartofPathology Research And Practicept_BR
dc.relation.ispartofabbreviationPathol. Res. Pract.pt_BR
dc.publisher.cityJenapt_BR
dc.publisher.countryAlemanhapt_BR
dc.publisherUrban & Fischer Verlagpt_BR
dc.date.issued2001pt_BR
dc.identifier.citationPathology Research And Practice. Urban & Fischer Verlag, v. 197, n. 10, n. 677, n. 682, 2001.pt_BR
dc.language.isoenpt_BR
dc.description.volume197pt_BR
dc.description.issuenumber10pt_BR
dc.description.firstpage677pt_BR
dc.description.lastpage682pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
unicamp.cruespUSPpt_BR
dc.identifier.issn0344-0338pt_BR
dc.identifier.wosidWOS:000171849800005pt_BR
dc.identifier.doi10.1078/0344-0338-00145pt_BR
dc.date.available2014-11-20T04:23:56Z
dc.date.available2015-11-26T16:05:34Z-
dc.date.accessioned2014-11-20T04:23:56Z
dc.date.accessioned2015-11-26T16:05:34Z-
dc.description.provenanceMade available in DSpace on 2014-11-20T04:23:56Z (GMT). No. of bitstreams: 1 WOS000171849800005.pdf: 617035 bytes, checksum: 0ca301c1bd98110ccdc7854072fa631e (MD5) Previous issue date: 2001en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:05:34Z (GMT). No. of bitstreams: 2 WOS000171849800005.pdf: 617035 bytes, checksum: 0ca301c1bd98110ccdc7854072fa631e (MD5) WOS000171849800005.pdf.txt: 21036 bytes, checksum: 1dd23cad177cf5fe4f2fb23ea9eb54ab (MD5) Previous issue date: 2001en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/73160pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/73160
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/73160-
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