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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleThe thiol-specific antioxidant enzyme prevents mitochondrial permeability transition - Evidence for the participation of reactive oxygen species in this mechanismpt_BR
dc.contributor.authorKowaltowski, AJpt_BR
dc.contributor.authorNetto, LESpt_BR
dc.contributor.authorVercesi, AEpt_BR
unicamp.authorUniv Estadual Campinas, Fac Ciencias Med, Dept Patol Clin, BR-13083970 Campinas, SP, Brazil Univ Estadual Campinas, Inst Biol, Dept Bioquim, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subject.wosPlus Prooxidantspt_BR
dc.subject.wosMembrane Permeabilizationpt_BR
dc.subject.wosInorganic-phosphatept_BR
dc.subject.wosHydrogen-peroxidept_BR
dc.subject.wosCross-linkingpt_BR
dc.subject.wosCa2+ Ionspt_BR
dc.subject.wosProteinpt_BR
dc.subject.wosReductasept_BR
dc.subject.wosHydroperoxidept_BR
dc.subject.wosPorespt_BR
dc.description.abstractMitochondrial swelling and membrane protein thiol oxidation associated with mitochondrial permeability transition induced by Ca2+ and inorganic phosphate are inhibited in a dose-dependent manner either by catalase, the thiol-specific antioxidant enzyme (TSA), a protein recently demonstrated to present thiol peroxidase activity, or ebselen, a selenium-containing heterocycle which also possesses thiol peroxidase activity. This inhibition of mitochondrial permeability transition is due to the removal of mitochondrial-generated H2O2, which can easily diffuse to the extramitochondrial space. Whereas ebselen required the presence of reduced glutathione as a reductant to grant its protective effect, TSA was fully reduced by mitochondrial components. Decrease in the oxygen concentration of the reaction medium also inhibits mitochondrial permeabilization and membrane protein thioloxidation, in a concentration-dependent manner. The results presented in this report confirm that mitochondrial permeability transition induced by Ca2+ and inorganic phosphate is reactive oxygen species-dependent. The possible importance of TSA as an intracellular antioxidant, avoiding the onset of mitochondrial permeability transition, is discussed in the text.pt
dc.relation.ispartofJournal Of Biological Chemistrypt_BR
dc.relation.ispartofabbreviationJ. Biol. Chem.pt_BR
dc.publisher.cityBethesdapt_BR
dc.publisher.countryEUApt_BR
dc.publisherAmer Soc Biochemistry Molecular Biology Incpt_BR
dc.date.issued1998pt_BR
dc.date.monthofcirculationMAY 22pt_BR
dc.identifier.citationJournal Of Biological Chemistry. Amer Soc Biochemistry Molecular Biology Inc, v. 273, n. 21, n. 12766, n. 12769, 1998.pt_BR
dc.language.isoenpt_BR
dc.description.volume273pt_BR
dc.description.issuenumber21pt_BR
dc.description.firstpage12766pt_BR
dc.description.lastpage12769pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0021-9258pt_BR
dc.identifier.wosidWOS:000073768500013pt_BR
dc.identifier.doi10.1074/jbc.273.21.12766pt_BR
dc.date.available2014-12-02T16:27:04Z
dc.date.available2015-11-26T16:33:43Z-
dc.date.accessioned2014-12-02T16:27:04Z
dc.date.accessioned2015-11-26T16:33:43Z-
dc.description.provenanceMade available in DSpace on 2014-12-02T16:27:04Z (GMT). No. of bitstreams: 1 WOS000073768500013.pdf: 576137 bytes, checksum: 66004dddb82f55afc42510f0f8e3a317 (MD5) Previous issue date: 1998en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:33:43Z (GMT). No. of bitstreams: 2 WOS000073768500013.pdf: 576137 bytes, checksum: 66004dddb82f55afc42510f0f8e3a317 (MD5) WOS000073768500013.pdf.txt: 26535 bytes, checksum: 904cbf1db3447c3a92c618129e5e730d (MD5) Previous issue date: 1998en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/72548pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/72548
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/72548-
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