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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePolyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli: Production and use in immunodiagnosispt_BR
dc.contributor.authorCalegario, RFpt_BR
dc.contributor.authorLocali, ECpt_BR
dc.contributor.authorStach-Machado, DRpt_BR
dc.contributor.authorPeroni, LApt_BR
dc.contributor.authorCaserta, Rpt_BR
dc.contributor.authorSalaroli, RBpt_BR
dc.contributor.authorFreitas-Astua, Jpt_BR
dc.contributor.authorMachado, MApt_BR
dc.contributor.authorKitajima, EWpt_BR
unicamp.authorCalegario, Renata F. Salaroli, Renato B. Kitajima, Elliot W. Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Fitopatol & Nematol, BR-13418900 Piracicaba, SP, Brazilpt_BR
unicamp.authorLocali, Eliane C. Caserta, Raquel Freitas-Astua, Juliana Machado, Marcos A. Ctr APTA Citros Sylvio Moreira, BR-13560970 Cordeiropolis, SP, Brazilpt_BR
unicamp.authorCaserta, Raquel Univ Estadual Campinas, Dept Anat Biol Celular Fisiol & Biofis, BR-13100000 Campinas, SP, Brazilpt_BR
unicamp.authorFreitas-Astua, Juliana EMBRAPA Mandioca & Fruticultura, Cruz Das Almas, BA, Brazilpt_BR
dc.subjectelectron microscopypt_BR
dc.subject.wosComplete Nucleotide-sequencept_BR
dc.subject.wosSpotted Wilt Viruspt_BR
dc.subject.wosGenomic Organizationpt_BR
dc.description.abstractThis work reports the in vitro expression of Citrus leprosis virus C (CiLV-C) putative coat protein (p29) and the production of a polyclonal antibody to be used as a tool for serological diagnosis of citrus leprosis. The ORF2/RNA1, corresponding to p29, was cloned in pET28a and transformed into Escherichia coli cells (BL21). Expression of p29 was induced in vitro and the protein was purified and used for immunization of rabbits to produce the polyclonal antibody. The anti p29 serum was shown to be highly specific to CiLV-C detection by immunological methods (Western blot, PTA-ELISA, tissue blot and in situ immunolocalization), without cross reaction with healthy citrus plants or other cytoplasmic and nuclear viruses transmitted by Brevipalpus mites. These results demonstrate that the antibody against CiLV-C p29 protein is highly specific for CiLV-C detection. In situ immunogold labeling assays on thin sections of sweet orange leaf cells infected by CiLV-C demonstrated that short, bacilliform particles present within cisternae of the endoplasmic reticulum were specifically labeled, confirming their viral nature. The dense cytoplasmic viroplasm was also heavily labeled indicating that it represents a site of p29
dc.relation.ispartofTropical Plant Pathologypt_BR
dc.relation.ispartofabbreviationTrop. Plant Pathol.pt_BR
dc.publisher.cityBrasilia D Fpt_BR
dc.publisherSoc Brasileira Fitopathologiapt_BR
dc.identifier.citationTropical Plant Pathology. Soc Brasileira Fitopathologia, v. 38, n. 3, n. 188, n. 197, 2013.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [2004/11799-0, 2008/52691-9]pt
dc.description.sponsordocumentnumberCNPq [47.1705/2009-8]pt
dc.description.provenanceMade available in DSpace on 2014-07-30T18:09:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:43:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
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