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Type: Artigo de periódico
Title: High-risk HPV detection by hybrid capture and ploidy determination by a computer-assisted system in cervical biopsies
Author: Shirata, NK
Espoladore, LMW
Filho, AL
Pittoli, JE
di Loreto, C
Pereira, SMM
Maeda, MYS
Utagawa, ML
Aguiar, LD
Roteli-Martins, C
Martinez, EG
Syrjanen, K
Abstract: OBJECTIVE: To correlate high-risk HPV (hrHPV) detection by Hybrid Capture II (HC2) (Digene, Gaithersburg, Maryland, U.S.A.) with DNA content (ploidy) of cervical biopsies analyzed by a computer-assisted system, STUDY DESIGN: Cervical biopsies from 54 women examined at Leonor Mendes de Barros Hospital, Sao Paulo, as part of the Latin American Screening study during 2002-2003, were tested for hrHPV with HC2. All patients had been referred for colposcopic examination due to an abnormal cervical cytology. The final diagnosis included 30 cervicitis, 14 cervical intraepithelial neoplasia (CIN) 1, 5 CIN 2, 4 CIN 3 and 1 squamous cell carcinoma (SCC). Five-micrometer sections of each biopsy were stained with Feulgen-tionine and evaluated with the CAS 200 System (Becton Dickinson, U.S.A.), using the3.0 software (version 8.1) of the DNA Quantitative Measurement Program (Becton Dickinson). Ploidy was evaluated from histograms obtained by analyzing atypical nuclei. RESULTS: 0 the 30 cervicitis cases, 28 (93.3%) were diploid, and hrHPV was detected in 8 (28.5%) of the cases. Two tetraploid cervicitis lesions were observed, 1 positive and 1 negative for hrHPV. Among the CIN 1 lesions, 8 (57.1%) were diploid and 6 (42.8%) aneuploid. Of the latter, 4 (66.6%) were negative and 2 (33.3%) positive for hrHPV. Of the 5 CIN 2 lesions, 2 were diploid, 2 aneuploid and 1 tetraploid; all were positive for hrHPV. All CIN 3 lesions and the SCC proved to be aneuploid and positive for hrHPV. CONCLUSION: The data suggest that the majority ofcervicitis and CIN 1 lesions are diploid and negative for hrHPV. This is in sharp contrast to high grade CIN 2-3 lesions, all of which were positive for hrHPV in this study and also aneuploid, consistent with their progressive potential.
Subject: cervical intraepithelial neoplasia
human papillomavirus
computer-assisted image analysis
image cytometry
Country: EUA
Editor: Sci Printers & Publ Inc
Citation: Analytical And Quantitative Cytology And Histology. Sci Printers & Publ Inc, v. 26, n. 5, n. 295, n. 300, 2004.
Rights: fechado
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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