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Type: Artigo de periódico
Title: Image preprocessing improves Fourier-based texture analysis of nuclear chromatin
Author: Adam, RL
Leite, NJ
Metze, K
Abstract: OBJECTIVE: To investigate whether preprocessing of digitized images can improve the image analysis of chromatin of cytologic preparations using Fast Fourier Transformation (FFT). STUDY DESIGN. In a preprocessing step the nuclear borders of the segmented nuclei were smoothed, thus avoiding the Airy ring artifact. We tested this method, comparing the inertia values of digitalized cardiomyocyte nuclei of rats of different ages. Furthermore, we created in silicio nuclear images with chromatin alterations at or nearby the nuclear edge in order to investigate the robustness of our method. RESULTS: After preprocessing, the FFT-derived variable inertia discriminated significantly better the chromatin structure of the nuclei at different ages in every frequency range. The investigation on simulated nuclei revealed that within the frequency ranges from 1.8 mu m to 0.72 mu m smoothing of the borders does not interfere with the detection of chromatin changes at the nuclear border. CONCLUSION: Smoothing of borders in segmented images can improve the analysis of Fourier-derived variables of the chromatin texture.
Subject: chromatin
Fourier analysis
image analysis
Country: EUA
Editor: Sci Printers & Publ Inc
Citation: Analytical And Quantitative Cytology And Histology. Sci Printers & Publ Inc, v. 30, n. 3, n. 175, n. 184, 2008.
Rights: fechado
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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