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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleIdentification of novel soybean microRNAs involved in abiotic and biotic stressespt_BR
dc.contributor.authorKulcheski, FRpt_BR
dc.contributor.authorde Oliveira, LFVpt_BR
dc.contributor.authorMolina, LGpt_BR
dc.contributor.authorAlmerao, MPpt_BR
dc.contributor.authorRodrigues, FApt_BR
dc.contributor.authorMarcolino, Jpt_BR
dc.contributor.authorBarbosa, JFpt_BR
dc.contributor.authorStolf-Moreira, Rpt_BR
dc.contributor.authorNepomuceno, ALpt_BR
dc.contributor.authorMarcelino-Guimaraes, FCpt_BR
dc.contributor.authorAbdelnoor, RVpt_BR
dc.contributor.authorNascimento, LCpt_BR
dc.contributor.authorCarazzolle, MFpt_BR
dc.contributor.authorPereira, GAGpt_BR
dc.contributor.authorMargis, Rpt_BR
unicamp.authorKulcheski, Franceli R. de Oliveira, Luiz F. V. Molina, Lorrayne G. Almerao, Mauricio P. Margis, Rogerio Univ Fed Rio Grande do Sul, Lab Genomes & Plant Populat, Ctr Biotechnol, BR-91501970 Porto Alegre, RS, Brazilpt_BR
unicamp.authorKulcheski, Franceli R. de Oliveira, Luiz F. V. Molina, Lorrayne G. Almerao, Mauricio P. Margis, Rogerio Univ Fed Rio Grande do Sul, Lab Genomes & Plant Populat, PPGBCM, BR-91501970 Porto Alegre, RS, Brazilpt_BR
unicamp.authorde Oliveira, Luiz F. V. Molina, Lorrayne G. Margis, Rogerio Univ Fed Rio Grande do Sul, PPGGBM, BR-91501970 Porto Alegre, RS, Brazilpt_BR
unicamp.authorRodrigues, Fabiana A. Marcolino, Juliana Barbosa, Joice F. Stolf-Moreira, Renata Nepomuceno, Alexandre L. Marcelino-Guimaraes, Francismar C. Abdelnoor, Ricardo V. EMBRAPA Soja, BR-86001970 Londrina, PR, Brazilpt_BR
unicamp.authorNascimento, Leandro C. Carazzolle, Marcelo F. Pereira, Goncalo A. G. Univ Estadual Campinas, Lab Genom & Express, Inst Biol, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorCarazzolle, Marcelo F. Univ Estadual Campinas, Natl Ctr High Performance Proc CENAPAD SP, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subject.wosGenome-wide Identificationpt_BR
dc.subject.wosSmall Rnaspt_BR
dc.subject.wosPlant Micrornaspt_BR
dc.subject.wosRegulated Micrornaspt_BR
dc.subject.wosDrought Stresspt_BR
dc.subject.wosTarget Genespt_BR
dc.description.abstractBackground: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families. Conclusions: Validation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit-and rust-stress
dc.relation.ispartofBmc Genomicspt_BR
dc.relation.ispartofabbreviationBMC Genomicspt_BR
dc.publisherBiomed Central Ltdpt_BR
dc.identifier.citationBmc Genomics. Biomed Central Ltd, v. 12, 2011.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberCNPq [140578/2009-9, 509828/2010-8, 303967/2008-0]pt
dc.description.sponsordocumentnumberCNPq [CNPq 5527/2007-8]pt
dc.description.sponsordocumentnumberCNPq [CNPq 559636/2009-1]pt
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