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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleIdentification of differentially transcribed genes in shrimp Litopenaeus vannamei exposed to osmotic stress and challenged with WSSV viruspt_BR
dc.contributor.authorGoncalves-Soares, Dpt_BR
dc.contributor.authorSeiffert, WQpt_BR
dc.contributor.authorSchlindwein, ADpt_BR
dc.contributor.authorToledo-Silva, Gpt_BR
dc.contributor.authorZanette, Jpt_BR
dc.contributor.authorMarques, MRFpt_BR
dc.contributor.authorBainy, ACDpt_BR
unicamp.authorGoncalves-Soares, Daniela Freire Marques, Maria Risoleta Dias Bainy, Afonso Celso Univ Fed Santa Catarina, LABCAI, NEPAQ, Dept Bioquim, BR-88034257 Florianopolis, SC, Brazilpt_BR
unicamp.authorSchlindwein, Aline Daiane Univ Fed Santa Catarina, Lab Protozool, Dept Microbiol & Parasitol, BR-88040900 Florianopolis, SC, Brazilpt_BR
unicamp.authorToledo-Silva, Guilherme Univ Estadual Campinas, Lab Anal Genet & Mol, Ctr Biol Mol & Engn Genet, Inst Biol,Dept Genet & Evolucao, BR-13083875 Campinas, SP, Brazilpt_BR
unicamp.authorZanette, Juliano Univ Fed Rio Grande, Inst Ciencias Biol, Lab Bioquim & Biol Mol, BR-96201900 Rio Grande, RS, Brazilpt_BR
dc.subjectLitopenaeus vannameipt_BR
dc.subjectOsmotic stresspt_BR
dc.subject.wosSuppression Subtractive Hybridizationpt_BR
dc.subject.wosC-type Lectinpt_BR
dc.subject.wosLipopolysaccharide-binding Proteinpt_BR
dc.subject.wosFresh-water Crayfishpt_BR
dc.subject.wosBlack Tiger Shrimppt_BR
dc.description.abstractThe effects of hyposmotic stress and white spot syndrome virus (WSSV) challenge in expression was studied in the marine shrimp Litopenaeus vannamei. Messenger RNA from gills of shrimp submitted to osmotic stress was isolated to identify genes differentially expressed through the suppressive subtractive hybridization (SSH) method. Two subtractive libraries forward and two reverse were constructed to identify up and down-regulated genes under these conditions. About 192 clones were sequenced, of which 46 genes were identified. These genes encode proteins corresponding to a wide range of biological roles, including defense, cell signaling, electron transfer, cell proliferation and differentiation, apoptosis, intermediary metabolism, cytoskeleton and digestion. Among the identified genes, 19 were up-regulated and 27 were down-regulated in the animals kept at a lower ion concentration. We evaluated the expression of eight genes by RT-qPCR in shrimp submitted to hyposmotic conditions with and without WSSV challenge. The SSH enabled the identification of genes that are influenced by hyposmotic stress. A significant up-regulation was observed in lectin-C, QM, TGF beta inducible nuclear protein 1, ciclophilin, malate dehydrogenase, mitochondrial ATP synthase F chain and ATP synthase subunit 9 precursor transcripts. However, the expression of these genes in L. vannamei was not affected by WSSV infection both at isosmotic and hyposmotic conditions. (C) 2011 Elsevier Inc. All rights
dc.relation.ispartofComparative Biochemistry And Physiology D-genomics & Proteomicspt_BR
dc.relation.ispartofabbreviationComp. Biochem. Physiol. D-Genomics Proteomicspt_BR
dc.publisher.cityNew Yorkpt_BR
dc.publisherElsevier Science Incpt_BR
dc.identifier.citationComparative Biochemistry And Physiology D-genomics & Proteomics. Elsevier Science Inc, v. 7, n. 1, n. 73, n. 81, 2012.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipFINEP-Brazil [01070625-00/336-2007]pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFINEP-Brazil [01070625-00/336-2007]pt
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