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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleExpression of alpha-hemoglobin stabilizing protein gene during human erythropoiesispt_BR
dc.contributor.authordos Santos, COpt_BR
dc.contributor.authorDuarte, ASSpt_BR
dc.contributor.authorSaad, STOpt_BR
dc.contributor.authorCosta, FFpt_BR
unicamp.author.emailferreira@unicamp.brpt_BR
unicamp.authorUniv Estadual Campinas, UNICAMP, Hemoctr, Ctr Hemotherapy & Hematol, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subject.wosQuantitative Pcr Analysispt_BR
dc.subject.wosChainspt_BR
dc.subject.wosCellspt_BR
dc.description.abstractalpha-Hemoglobin stabilizing protein (AHSP) is an abundant, erythroid-specific protein that forms a stable complex with free alpha-hemoglobin but not with beta-hemoglobin or hemoglobin A. As such, AHSP is required for normal erythropoiesis, probably acting by blocking the deleterious effects of free alpha-hemoglobin precipitation. In order to study the levels of expression of the AHSP gene during the different phases of erythropoiesis, we carried out a two-phase liquid culture of erythroid cells and real-time quantitative polymerase chain reaction. Blood from control volunteers was cultured with erythropoietin to stimulate differentiation. The different stages of erythropoiesis were confirmed by morphologic and flow cytometric analysis. The results showed a progressive increase in AHSP gene expression following the expression of alpha-globin gene, during maturation of the red blood cell precursors, confirming the probable important function of this protein during normal erythropoiesis. (C) 2004 International Society for Experimental Hematology. Published by Elsevier Inc.pt
dc.relation.ispartofExperimental Hematologypt_BR
dc.relation.ispartofabbreviationExp. Hematol.pt_BR
dc.publisher.cityNew Yorkpt_BR
dc.publisher.countryEUApt_BR
dc.publisherElsevier Science Incpt_BR
dc.date.issued2004pt_BR
dc.date.monthofcirculationFEBpt_BR
dc.identifier.citationExperimental Hematology. Elsevier Science Inc, v. 32, n. 2, n. 157, n. 162, 2004.pt_BR
dc.language.isoenpt_BR
dc.description.volume32pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage157pt_BR
dc.description.lastpage162pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0301-472Xpt_BR
dc.identifier.wosidWOS:000220965500005pt_BR
dc.identifier.doi10.1016/j.exphem.2003.11.002pt_BR
dc.date.available2014-11-18T03:19:59Z
dc.date.available2015-11-26T17:45:48Z-
dc.date.accessioned2014-11-18T03:19:59Z
dc.date.accessioned2015-11-26T17:45:48Z-
dc.description.provenanceMade available in DSpace on 2014-11-18T03:19:59Z (GMT). No. of bitstreams: 1 WOS000220965500005.pdf: 359188 bytes, checksum: 9f46f7799f4fff7794afb3c4c904110c (MD5) Previous issue date: 2004en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:45:48Z (GMT). No. of bitstreams: 2 WOS000220965500005.pdf: 359188 bytes, checksum: 9f46f7799f4fff7794afb3c4c904110c (MD5) WOS000220965500005.pdf.txt: 21871 bytes, checksum: 523818a6a9768fa3361bcbaa8e00aade (MD5) Previous issue date: 2004en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66461pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/66461
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66461-
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