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Type: Artigo de periódico
Title: Diabetes modulates gene expression in the gingival tissues of patients with chronic periodontitis
Author: Duarte, PM
Neto, JBC
Casati, MZ
Sallum, EA
Nociti, FH
Abstract: Aim This study evaluated whether diabetes modulates gene expression [interleukin (IL)-1 beta, IL-1ra, IL-6, IL-8, IL-10; tumor necrosis factor (TNF)-alpha; interferon (IFN)-gamma, receptor activator of NF-kappa B ligand (RANKL) and osteoprotegerin (OPG)] in sites with periodontitis. Materials and Methods Gingival biopsies were harvested and divided into three groups - Control group: systemically and periodontally healthy subjects (n = 10); Periodontitis group: systemically healthy subjects diagnosed with chronic periodontitis (n = 20); Diabetes group: type 1 diabetic subjects, diagnosed with chronic periodontitis (n = 20). Total RNA was obtained and analyzed by quantitative polymerase chain reaction. Results Data analysis demonstrated that, except for OPG, mRNA levels for all factors were increased by inflammation (P < 0.001). Interleukin-1 beta, IL-1ra, IL-6, IL-8, IFN-gamma, and RANKL mRNA levels were higher in the diabetic group when compared with the control non-periodontitis group (P < 0.05), whereas IL-10 and OPG were lower (P < 0.05). No difference was observed for TNF-alpha between diabetic and control groups (P > 0.05). Diabetes lowered IL-1 beta, IL-8, IL-10, TNF-alpha, RANKL, and OPG mRNA levels in sites with comparable type of periodontitis (P < 0.001). Moreover, increased RANKL:OPG and IL-6:IL-10 ratios were found. Conclusions Taken together, these data suggest that decreased levels of IL-10 and OPG may play an important role in the periodontal breakdown in diabetic patients.
Subject: cytokines
periodontal disease
gene expression
Country: Inglaterra
Editor: Blackwell Publishing
Citation: Oral Diseases. Blackwell Publishing, v. 13, n. 6, n. 594, n. 599, 2007.
Rights: fechado
Identifier DOI: 10.1111/j.1601-0825.2006.01348.x
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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