Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Determination of propranolol enantiomers in plasma and urine by spectrofluorimetry and second-order standard addition method
Author: Valderrama, P
Poppi, RJ
Abstract: The determination of propranolol enantiomers in human plasma and urine by spectrofluorimetry and a second-order standard addition method is described. The methodology is based on chiral recognition of propranolol by formation of an inclusion complex with beta-cyclodextrin, a chiral auxiliary, in the presence of I-butanol. The adopted strategy combines the use of PARAFAC, for extraction of the pure analyte signal, with the standard addition method, for determinations in the presence of an individual matrix effect caused by the quenching action of the proteins present in the plasma and urine. A specific PARAFAC model was built for each sample, in triplicate, and the scores were related to (R)-propranolol mole fraction using a linear regression in the standard addition method. Using a propranolol with concentration of 260 ng mL(-1), good results were obtained for determinations in the mole fraction range from 50 to 80% of (R)-propranolol, providing absolute errors between 0.4 and 3.6% for plasma and between 0.9 and 6.0% for urine. (C) 2009 Elsevier B.V. All rights reserved.
Subject: Propranolol
Human plasma
Second-order standard addition method
Country: Holanda
Editor: Elsevier Science Bv
Citation: Analytica Chimica Acta. Elsevier Science Bv, v. 651, n. 1, n. 31, n. 35, 2009.
Rights: fechado
Identifier DOI: 10.1016/j.aca.2009.08.003
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000270104800004.pdf472.09 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.