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Type: Artigo de periódico
Title: LaTBP1: A Leishmania amazonensis DNA-binding protein that associates in vivo with telomeres and GT-rich DNA using a Myb-like domain
Author: Lira, CBB
Neto, JLD
Khater, L
Cagliari, TC
Peroni, LA
dos Reis, JRR
Ramos, CHI
Cano, MIN
Abstract: Different species of Leishmania can cause a variety of medically important diseases, whose control and treatment are still health problems. Telomere binding proteins (TBPs) have potential as targets for anti-parasitic chemotherapy because of their importance for genome stability and cell viability. Here, we describe LaTBP1 a protein that has a Myb-like DNA-binding domain, a feature shared by most double-stranded telomeric proteins. Binding assays using full-length and truncated LaTBP1 combined with spectroscopy analysis were used to map the boundaries of the Myb-like domain near to the protein only tryptophan residue. The Myb-like domain of LaTBP1 contains a conserved hydrophobic cavity implicated in DNA-binding activity. A hypothetical model helped to visualize that it shares structural homology with domains of other Myb-containing proteins. Competition assays and chromatin immunoprecipitation confirmed the specificity of LaTBP1 for telomeric and GT-rich DNAs, suggesting that LaTBP1 is a new TBP. (C) 2007 Elsevier Inc. All rights reserved.
Subject: double-stranded DNA-binding protein
GT-rich DNA
hydrophobic cavity
leishmania amazonensis
myb-like domain
Country: EUA
Editor: Elsevier Science Inc
Citation: Archives Of Biochemistry And Biophysics. Elsevier Science Inc, v. 465, n. 2, n. 399, n. 409, 2007.
Rights: fechado
Identifier DOI: 10.1016/
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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