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Type: Artigo de periódico
Title: Increased L-CPT-1 activity and altered gene expression in pancreatic islets of malnourished adult rats: a possible relationship between elevated free fatty acid levels and impaired insulin secretion
Author: Reisa, MAD
Arantes, VC
Cunha, DA
Latorraca, MQ
Toyama, MH
Carneiro, EM
Boschero, AC
Abstract: Intrauterine growth restriction is associated with chronically elevated levels of serum fatty acids and reduced glucose-stimulated insulin secretion. Lipid metabolism in pancreatic p cells is critical for the regulation of insulin secretion, and the chronic exposure to fatty acids results in higher palmitate oxidation rates and an altered insulin response to glucose. Using a rat model of isocaloric protein restriction, we examined whether pre- and postnatal protein malnutrition influences the properties of pancreatic islet carnitine palmitoyltransferase-1 (liver isoform, L-CPT-1), a rate-limiting enzyme that regulates fatty acid oxidation in mitochondria. The activity of L-CPT-1 in pancreatic islets increased in the low protein (LP), although the L-CPT-1 mRNA levels were unaffected by malnutrition. The susceptibility of enzyme to inhibition by malonyl-CoA was unaltered and the content of malonyl-CoA was reduced in LP cells. Because the mitochondrial oxidation of fatty acids is related to the altered expression of a number of genes encoding proteins involved in insulin secretion, the levels of expression of insulin and GLUT-2 mRNA were assessed. A reduced expression of both genes was observed in malnourished rats. These results provide further evidence that increased L-CPT-1 activity and changes in gene expression in pancreatic islets may be involved in the reduced insulin secretion seen in malnourished rats. (c) 2008 Published by Elsevier Inc.
Subject: insulin secretion
low-protein diet
fatty acids
Country: EUA
Editor: Elsevier Science Inc
Citation: Journal Of Nutritional Biochemistry. Elsevier Science Inc, v. 19, n. 2, n. 85, n. 90, 2008.
Rights: fechado
Identifier DOI: 10.1016/j.jnutbio.2007.01.005
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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