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Type: Artigo de periódico
Title: Quantifying loss of CD34(+) cells collected by apheresis after processing for freezing and post-thaw
Author: Castelhano, MV
Reis-Alves, SC
Vigorito, AC
Rocha, FF
Pereira-Cunha, FG
De Souza, CA
Lorand-Metze, I
Abstract: Introduction: CD34(+) cells collected for autologous bone marrow transplantation (BMT) are usually quantified in the apheresis product after collection, but the necessity to repeat these measures post-thaw is controversial. Methods: We examined the loss of CD34(+) cells after collection, preparation for freezing and post-thaw in apheresis products collected for BMT. Results: Median number of CD34(+) cells collected per unit was 1.61 x 10(6)/kg, viability: 97-100%. This number decreased to 1.38 x 10(6)/kg, viability: 96-100% before freezing and was 1.17 x 10(6)/kg post-thaw. Viability decreased to 86-98%. The relative loss of viable PBHPC showed an inverse correlation with the ratio "CD34(+) cells/total nucleated cells" (r = -0.45; p = <0.0005). This relative loss was largest in patients with Hodgkin's lymphoma. Conclusion: Cryopreservation and thawing of PBHPCs in leukapheresis products provokes a small but significant stem cell loss. So, quantification of viable CD34(+) cells post-thaw is important, especially in poorly mobilizing patients. Besides, the ratio "CD34(+) cells/total nucleated cells" after leukapheresis is an important parameter for prediction of neutrophil recovery after BMT. (c) 2013 Elsevier Ltd. All rights reserved.
Subject: CD34(+) cells
Bone marrow transplantation
Quality control
Flow cytometry
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Citation: Transfusion And Apheresis Science. Pergamon-elsevier Science Ltd, v. 48, n. 2, n. 241, n. 246, 2013.
Rights: fechado
Identifier DOI: 10.1016/j.transci.2013.01.017
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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