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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleMolecular detection of Bacteroides forsythus in infested root canalspt_BR
dc.contributor.authorGoncalves, RBpt_BR
dc.contributor.authorMouton, Cpt_BR
unicamp.authorUNICAMP, Fac Odontol Piracicaba, Dept Diagnost Oral, Piracicaba, SP, Brazil Univ Laval, Fac Med Dent, Grp Rech Ecol Buccale, Quebec City, PQ, Canadapt_BR
dc.subject.wosDna-probe Assaypt_BR
dc.subject.wosAdvanced Periodontitispt_BR
dc.subject.wosSubgingival Plaquept_BR
dc.subject.wosIdentificationpt_BR
dc.subject.wosBacteriapt_BR
dc.subject.wosPcrpt_BR
dc.subject.wosGingivalispt_BR
dc.subject.wosPocketspt_BR
dc.subject.wosTeethpt_BR
dc.subject.wosFlorapt_BR
dc.description.abstractThe purpose of the present study was to investigate the occurrence of Bacteroides forsythus in infections of dental root canals. Eleven samples from infected root canals were analyzed by four different molecular methods. The prevalence of the monitored species varied as a function of the detection method. The polymerase chain reaction DNA probe method after immunocapture yielded the highest prevalence value (6/11), whereas the lowest value was observed with the slot-blot (3/11), Of the 11 canal samples, 5 were positive by ELISA and 4 were positive by immunofluorescence. The presence of B. forsythus was detected by all four methods in 3/11 canals, whereas 4/11 appeared to be free of B. forsythus, Our data indicate that B. forsythus can be part of the endodontic microflora, The procedure consisting of immunomagnetic capture and a polymerase chain reaction-DNA probe assay can be useful as an alternative to culture for clinical studies of the species infecting human dental pulp.pt
dc.relation.ispartofJournal Of Endodonticspt_BR
dc.relation.ispartofabbreviationJ. Endod.pt_BR
dc.publisher.cityPhiladelphiapt_BR
dc.publisher.countryEUApt_BR
dc.publisherLippincott Williams & Wilkinspt_BR
dc.date.issued1999pt_BR
dc.date.monthofcirculationMAYpt_BR
dc.identifier.citationJournal Of Endodontics. Lippincott Williams & Wilkins, v. 25, n. 5, n. 336, n. 340, 1999.pt_BR
dc.language.isoenpt_BR
dc.description.volume25pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage336pt_BR
dc.description.lastpage340pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0099-2399pt_BR
dc.identifier.wosidWOS:000080030700005pt_BR
dc.identifier.doi10.1016/S0099-2399(06)81167-2pt_BR
dc.date.available2014-12-02T16:24:21Z
dc.date.available2015-11-26T17:35:50Z-
dc.date.accessioned2014-12-02T16:24:21Z
dc.date.accessioned2015-11-26T17:35:50Z-
dc.description.provenanceMade available in DSpace on 2014-12-02T16:24:21Z (GMT). No. of bitstreams: 1 WOS000080030700005.pdf: 695093 bytes, checksum: ddd01b06cb92b7abea9b478eb5ecb5bb (MD5) Previous issue date: 1999en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:35:50Z (GMT). No. of bitstreams: 2 WOS000080030700005.pdf: 695093 bytes, checksum: ddd01b06cb92b7abea9b478eb5ecb5bb (MD5) WOS000080030700005.pdf.txt: 27600 bytes, checksum: 3f6ee5d6110bcfcc368983a41d5da69c (MD5) Previous issue date: 1999en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57750pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/57750
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57750-
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